Mutational analysis of complementary-sense genes of African cassava mosaic virus DNA A

RNA-primed complementary-sense DNA synthesis of the geminivirus African cassava mosaic virus.

The plant DNA virus African cassava mosaic virus (ACMV) is believed to replicate by a rolling circle mechanism. To investigate complementary-sense DNA (lagging strand) synthesis, we have analysed the heterogenous form of complementary-sense DNA (H3 DNA) from infected Nicotiana benthamiana by two-dimensional agarose gel electrophoresis and blot hybridisation. The presence of an RNA moeity is dem...

Geminate structures of African cassava mosaic virus.

Two types of geminate structures were purified from African cassava mosaic geminivirus (ACMV)-infected Nicotiana benthamiana plants and analyzed by electron cryomicroscopy and image reconstruction. After cesium sulfate density gradient centrifugation, they were separated into lighter top (T) and heavier bottom (B) components. T particles comigrated with host proteins, whereas B particles were c...

Regulation of African cassava mosaic virus complementary-sense gene expression by N-terminal sequences of the replication-associated protein AC1.

Fragments of the African cassava mosaic virus (ACMV) genome, cloned upstream of the beta-glucuronidase (GUS) reporter gene in an expression cassette, were analysed for their ability to direct complementary-sense gene expression in tobacco protoplasts by measuring GUS activity. Five arbitrary domains (A-E) have been designated that contribute to the expression of AC1 (replication-associated prot...

Occurrence of African cassava mosaic virus (ACMV) and East African cassava mosaic virus – Uganda (EACMV-UG) in Jatropha curcas

Background Jatropha curcas is a drought resistant shrub native in tropical America, now widely grown in many tropical and subtropical regions for biodiesel production [1]. First reports on virus infections in Jatropha indicated the occurrence of viruses closely related to Cassava mosaic virus in India, reaching a disease incidence from 25 to 47%. This might represent a major constrain to the pr...

Nucleotide sequence of the infectious cloned DNA components of African cassava mosaic virus (Nigerian strain)